Nagzira has diverse type of vegetation ranging from dry mixed forests to moist forest. The forest type is Southern Tropical Dry Deciduous Forests with lakes spread all around the area. The animals commonly spotted are Tigers, Panthers, Leopards, Bisons, Sloth Bears, Sambars, Four-Headed Antelopes, Blue Bulls, Chitals, Barking Deers, Mouse Deers, Civet Cats, Jackals, Jungle Cats, Spotted Hyenas, Wild Dogs and Hares. Among the birds that are prominent and commonly seen are Peafowl, the Grey Jungle Fowl and the Red Spur Fowl. The habitats of sanctuary include 34 species of mammals, 170 species of birds, 36 species of reptiles, 4 species of amphibians, and number of fishes. The invertebrate fauna includes, besides a number of insects and ant species, 49 species of butterflies.

Babblers, Serpant Eagle, Gesse, Guls, Warblers, Pipits, Chats, Sunbirds, Larks, Kingfisher, Owls, Imperial Eagle, Magpie Robin, Brahmani Kite, Indian Pitta, Horn bills, Red Whiskered Bulbuls, Rocket tail drongo, Black Drongo, Black and White kingfisher, Common Green Pegion, The great Indian Bustard.